CJC-1295 (No DAC) — Research Peptide CJC-1295 (No DAC)—also known as Modified GRF (1-29)—is a 29-amino-acid analog of growth-hormone-releasing hormone (GHRH). The “No DAC” version does not include a Drug Affinity Complex; instead, it uses four sequence substitutions designed to improve stability while maintaining short, pulsatile kinetics characteristic of GRF(1-29). Key Points Class: GHRH analog (29 aa) studied for GH/IGF-1 axis modulation and pulsatile GH release. Stability substitutions (tetra-substituted): D-Ala2, Gln8, Ala15, Leu27 (engineered to resist enzymatic breakdown such as DPP-4). No DAC: Lacks the albumin-binding “DAC” moiety; retains shorter active window vs. DAC-linked variants. Mechanistic focus: GHRH-receptor engagement on pituitary somatotrophs → cAMP/PKA signaling → GH secretory pulses (model-dependent). Research Highlights (Preclinical/Exploratory) GH/IGF-1 axis: Studies on modified GRF(1-29) report increased pulsatile GH output and IGF-1 changes under defined protocols; outcomes vary by model, dose, and sampling window. Tissue readouts: Exploratory work notes signal-dependent changes in lean-mass proxies and skin-thickness measures; mechanistic attribution remains under investigation. GI smooth muscle: GHRH-analog interactions with VPAC1-linked pathways have been explored in gut motility models (protocol-specific). Cardiac models: GHRH-agonist peptides have been studied post-ischemia in rodents for ejection-fraction and tissue-repair markers (class-level findings; model-dependent). Combinations: Frequently paired with GHS/ghrelin-receptor agonists (e.g., ipamorelin) to probe dual-pathway GH stimulation (GHRH + GHSR) and potential synergy. Chemical Information Molecular Formula: C152H252N44O42 Molecular Weight: 3367.9 g/mol Also Known As: CJC-1295 without DAC; Modified GRF (1-29) Specifications Form: Lyophilized powder Purity: ≥99% (HPLC); COA available per lot Packaging: Research-grade, sealed vial Storage & Handling Store lyophilized material in a cool, dry place, protected from light. After reconstitution: refrigerate at 2–8 °C. For long-term stability: store at −20 °C; avoid repeated freeze–thaw cycles. Compliance Notice For laboratory research use only. Not for human or veterinary use. No medical claims are made; descriptions refer to in vitro/in vivo research contexts.